Structural analysis of H+-translocating inorganic pyrophosphatase

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Proton-translocating inorganic pyrophosphatase is an electrogenic proton pump that uses inorganic pyrophosphate as the substrate. The members of this protein family are widely distributed in most plants, but only some protozoa, bacteria, and archaebacteria. Although its importance in membrane transport systems, its coupling mechanism between the pyrophosphate hydrolysis and the active proton transport is still unknown. Here, structural study of the plant-derived enzyme was carried out.