An electron microscopic study of actin nucleation factors

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The actin filament is responsible for a wide range of movement of the cell and dynamic through construction and destruction. Many proteins regulate the dynamics of the actin filament. Arp2/3 complex and formin are ones of the regulatory proteins and they start the construction of the actin filament in the cell. To understand mechanism of starting construction, we developed structural analysis procedures of the actin filament network in the cell with an Austrian group, which determined distribution and structure of Arp2/3 complex in the cell by using electron microscopy and image analysis. We also elucidated that formin binds only to the ends of the actin filament, not the side. Now we are analyzing data to conform the bound end to formin is the barbed end.