A genetical screening of a protein trap library for active chromatin-binding factors on polytene chromosome in Drosophila

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It is evident that gene regulation is coupled with chromatin reorganaization, but factors and molecular basis involving in this process remain to be identified. In the present study, factors responsible for ecdysone-induced puff formation were genetically screened in Drosophila fly by establishing an EGFP protein-trap approach. Among the candidates, Drosophila CG11138/Ringfect was found as a co-activator for ecdysone receptor (EcR). Through biochemical characterization, Ringfect could be shown to serve as a histone chaperon. We describe the identification of novel puff-localized factors by screening the protein trap transgenic fly library. Drosophila CG11138/Ringfect and enolase were genetically identified as a puff-localized factor and a co-activator, and interacted with EcR in S2 cell. Thus, the present study suggests that Ringfect and enolase are categorized as a novel class of nuclear receptor co-activators.