Searching for parthenocarpic-related genes in grape by next-generation sequencing technology

利用二代测序技术寻找葡萄单性结实相关基因

• 批准号: 22780021
• 负责人: HABU Tsuyoshi
• 金额: $ 2.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780021/
• 关键词: 果樹園芸; ブドウ; EST解析; 単為結果; ジベレリン; 果樹園芸学; 熱ショックタンパク質

In this study, to elucidate the mechanism of gibberellic acid induced parthenocarpy, I searched for possible parthenocarpy-related genes by next-generation sequencing technology. By large-scale sequencing analyses in non-treated and GA-treated grape fruits, the expression of heat chock protein(HSP) genes was significantly reduced after GA treatment, indicating that the HSPs may be related to the GA-induced parthenocarpy in grape.

为了阐明赤霉素诱导单性结实的机制，本研究利用新一代测序技术寻找可能与单性结实相关的基因。通过对未处理和GA处理的葡萄果实进行大规模测序分析，发现GA处理后热激蛋白（heat chock protein，HSP）基因的表达量显著降低，表明HSP基因可能与GA诱导的葡萄单性结实有关。