Analysis of gene expression in the cells isolated by laser capture microdissection from different petal tissues

激光捕获显微切割不同花瓣组织分离细胞的基因表达分析

• 批准号: 22780030
• 负责人: HARADA Taro
• 金额: $ 2.5万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780030/
• 关键词: 花卉; カーネーション; 開花; 遺伝子発現; 遺伝子; 園芸学; 花き; マクロアレイ

Cells from epidermis, parenchyma and vascular bundles were isolated from carnation petals by laser capture microdissection and differential gene expression among these petal tissues was demonstrated. In addition, differential gene expression between adaxial and abaxial sides of parenchyma in a bending zone of carnation petals was revealed by macroarray analysis. One gene encoding lipid transfer protein(DcLTP1) showed relatively high expression level in blade epidermis, while its expression was higher in the adaxial side of parenchyma from the bending zone.

通过激光捕获显微解剖和这些花瓣组织中的差异基因表达，从表皮，实质和血管束从康乃馨花瓣中分离出来。此外，通过大型阵列分析揭示了实质弯曲区域中实质的近距离和亚障边之间的差异基因表达。一个编码脂质转移蛋白（DCLTP1）的基因在叶片表皮中显示出相对较高的表达水平，而其表达在弯曲区的实质的近端侧较高。