Unraveling of interaction between Magnaporthe oryzae effectors and host targets during rice blast infection.

我们成功地从稻瘟病菌中鉴定出三个无毒效应因子AVR-Pia、AVR-PII、AVR-Pik和一个致病蛋白MC69。此外，还分离到了水稻抗稻瘟病(R)基因Pia。随后我们发现，MC69是一种分泌性蛋白，是单子叶和双子叶真菌分别侵染水稻根结线虫和黄瓜炭疽菌所必需的。我们还揭示了R蛋白Pik1通过蛋白质-蛋白质直接相互作用识别AVR-Pik。此外，还成功分离到了另一个R基因PII。

We have successfully identified three avirulence effectors AVR-Pia, AVR-Pii, AVR-Pik and a pathogenicity protein MC69 from the rice blast fungus Magnaporthe oryzae. In addition, a rice blast resistance (R) gene Pia has been isolated. We have then found out that MC69 is a secreted protein and required for infection by monocot and dicot fungal pathogens, M. oryzae and cucumber anthracnose fungus Colletotrichum orbiculare, respectively. We also revealed that an R protein Pik1 recognizes AVR-Pik through direct protein-protein interaction. Furthermore, another R gene Pii has been successfully isolated.