Analysis of the origin and the mechanism of persistent infection of BmMLV

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To identify the origin of Bombyx mori macula-like virus, we analyzed the host range of BmMLV. Several insect-and mammalian-derived cell lines were inoculated with BmMLV and analyzed for the propagation of BmMLV. We showed that only B. mori-derived cell lines were permissive to BmMLV persistent infection. Also, BmMLV-negative BmVF cells were inoculated with BmNPV. We identified that the recombinant protein was fully expressed and suitable post-translational modification was occurred in BmNPV-infected BmVF cells. These data showed that BmMLV replicates only in B. mori-derived cells and BmVF cell line was suitable for baculovirus expression vector system.