Genetic analysis of chemotactic receptors in bacteria

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We analyzed the mechanism of chemotaxis of Pseudomonas fluorescens strain KU-7 toward 2-nitrobenzoate. We observed that the chemotaxis of strain KU-7 to 2-nitrobenzoate is metabolism-dependent and that the chemoreceptor gene nbaY is induced by growth on 2-nitrobenzoate. Furthermore, the genes responsible for 2-nitrobenzoate metabolism, including nbaY, were found to be induced by the metabolic intermediate 2-amino-3- carboxymuconate-6-semialdehyde rather than 2-nitrobenzoate.