Analysis of cellular function of tRNase Z in Saccharomyces cerevisiae

核糖核酸内切酶tRNase Z在真核生物中通过特异性的鉴别位点内溶裂解催化tRNA前体成熟的3'端。在体外研究中，酵母tRNase Z蛋白具有前体tRNA 3'加工活性。酵母tRNase Z蛋白定位于细胞核，是生长所必需的。酵母tRNase Z的温度敏感（Ts）突变体表现出3 ' -未加工的前trna的积累。因此，我们的研究结果表明，sctRNase ZL可能是前trna的3 '端加工所必需的。

Endoribonuclease tRNase Z catalyzes the generation of the mature 3' end of tRNA precursors through specific endonucleolytic cleavage at discriminator site in eukaryotic organisms. The budding yeast Saccharomyces cerevisiae has a single long form of tRNase Z. The recombinant yeast tRNase Z protein possesses precursor tRNA 3'-processing activity in vitro. The yeast tRNase Z protein localizes to the nucleus and is essential for growth. Temperature-sensitive(Ts) mutants of yeast tRNase Z exhibit the accumulation of 3′-unprocessed pre-tRNAs. Thus, our findings suggest that the sctRNase ZL may be required for the 3′end processing of pre-tRNAs.