Characterization and structural analysis of novel dye-linked L-proline dehydrogenase

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Until now, two different types of Dye-ProDHs have been discovered in hyperthermophile. We have found the third novel type of Dye-ProDH whose molecular composition and properties were completelydifferent. Structure determination was performed. As the result, we succeeded the structural determination at high resolution. In addition, we succeeded the identification of the amino acid which is involved in substrate affinity and electron-transfer of the enzyme.