Molecular and developmental genetics of metamorphosis in fish

本研究旨在积累鱼类变态的基本科学知识，以帮助建立更有效的比目鱼养殖生产，这在日本具有巨大的经济影响。我以青鳉为模型进行了分子和发育遗传学研究。产生GFP转基因系以监测活鱼体内甲状腺激素水平。在另一种建立青鳉作为研究变态的模型系统的方法中，克隆了约20个与甲状腺大发育和/或甲状腺激素信号传导有关的候选标记基因，并检查了它们的胚胎表达模式。

This study aimed to accumulate the basic scientific knowledge on the metamorphosis in fish to help establishing more efficient production of the flounder aquaculture which has a huge economical impact in Japan. I conducted molecular and developmental genetic studies using medaka as a model. GFP transgenic lines were generated in order to monitor in vivo thyroid hormone level in a live fish. In another approach to establish medaka as a model system for studying metamorphosis, approximately 20 candidate marker genes that are implicated in thyroid grand development and/or thyroid hormone signaling were cloned and their embryonic expression patterns were examined.