Protective immunity against intracellularbacteria infish

为了阐明细胞介导的免疫和/或体液免疫是否有助于保护鱼类免受迟缓爱德华氏菌感染，我们检测了T细胞相关基因表达，特异性抗体滴度，细菌清除和免疫淋巴细胞亚群的过继转移。提示细胞免疫在大肠杆菌的免疫保护中起重要作用。鱼类迟发性感染此外，细胞毒性T淋巴细胞在保护鱼类免受E。随后，我们从黄尾鱼中克隆了T细胞相关基因。以这些基因为标记，对分枝杆菌病灭活疫苗的疫苗效力进行了评价。佐剂灭活疫苗诱导的细胞免疫应答，虽然灭活细胞增强了黄尾鱼的体液免疫。

To elucidate whether cell-mediated immunity and/or humoral immunity contribute to protection against Edwardsiella tardainfection in fish, we examined T-cell related gene expression, specific antibody titers, bacterial clearance, and adoptive transfer of immunized lymphocyte subsets. Our results indicate that cell mediated immunity play an important roles in the protection against E. tardainfection in fish. In addition, cytotoxic T lymphocytes are crucial player in protecting fish from E. tardainfection.Subsequently, we have cloned T-cell related genes from yellowtail. Using these genes as a marker, vaccine efficacy of inactivated vaccine against mycobacteriosis was evaluated. Inactivated vaccine with adjuvant induced cell-mediated immune response, although inactivated cells enhanced humoral immunity in yellowtail.