Protective immunity against intracellularbacteria infish

为了阐明细胞免疫和/或体液免疫是否有助于对鱼类迟缓爱德华氏菌感染的保护，我们检测了T细胞相关基因的表达、特异性抗体效价、细菌清除和免疫淋巴细胞亚群的过继转移。我们的结果表明，细胞免疫在鱼对迟发性埃希氏菌感染的保护中起着重要作用。此外，细胞毒性T淋巴细胞在保护鱼类免受迟发性埃希氏菌感染方面起着至关重要的作用。以这些基因为标志，对分枝杆菌病灭活疫苗的免疫效果进行了评价。虽然灭活细胞增强了黄尾鱼的体液免疫，但佐剂灭活疫苗诱导了细胞免疫反应。

To elucidate whether cell-mediated immunity and/or humoral immunity contribute to protection against Edwardsiella tardainfection in fish, we examined T-cell related gene expression, specific antibody titers, bacterial clearance, and adoptive transfer of immunized lymphocyte subsets. Our results indicate that cell mediated immunity play an important roles in the protection against E. tardainfection in fish. In addition, cytotoxic T lymphocytes are crucial player in protecting fish from E. tardainfection.Subsequently, we have cloned T-cell related genes from yellowtail. Using these genes as a marker, vaccine efficacy of inactivated vaccine against mycobacteriosis was evaluated. Inactivated vaccine with adjuvant induced cell-mediated immune response, although inactivated cells enhanced humoral immunity in yellowtail.