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Identification of disease resistance loci against bacterial disease (Streptococcus iniae)in rainbow trout(Oncorhynchus mykiss)

虹鳟鱼（Oncorhynchus mykiss）细菌性病害（海豚链球菌）抗病位点的鉴定

基本信息

批准号：
22780188
负责人：
OZAKI Akiyuki
金额：
$ 2.58万
依托单位：
Fisheries Research Agency
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Streptococcal disease (streptococcosis) caused by Streptococcus iniae is a serious bacterial disease in rainbow trout (Oncorhynchus mykiss). Developing streptococcosis-resistant rainbow trout will reduce the number of outbreaks of this disease as well as reduce the need for medicines and vaccines. Genetic linkage analysis is an effective method for identifying quantitative trait loci (QTL) associated with resistance to a disease. In this study, microsatellite markers selected from genetic linkage maps of rainbow trout and BC_1 progeny from crosses between disease-resistant and disease-susceptible parents were used for detection of QTL associated with resistance to this disease. The immune-related genes on the same linkage group are correlated with significant QTL about disease resistance. Especially some studies have reported that QTLs are located on the same linkage group with the MHC class I, II or toll-like receptor regions. Linkage groups on QTL region were specifically targeted in these studies because they carry the classical MHC class I and II genes previously linked to differences in susceptibility to viral, bacterial, and parasitic diseases. Some loci associated with disease resistance were found in the RT-3、RT-16、RT-2、RT-29、RT-27、RT-31、RT-17and RT-22 linkage groups. These QTL regions are candidates for disease resistance against streptococcal infection.

由牛链球菌引起的链球菌病是虹鳟（Oncorhynchus mykiss）中一种严重的细菌性疾病。培育具有链球菌抗性的虹鳟鱼将减少这种疾病爆发的次数，并减少对药物和疫苗的需求。遗传连锁分析是鉴定抗病相关数量性状位点的有效方法。本研究从虹鳟鱼抗病亲本和病感亲本杂交的BC_1后代遗传连锁图谱中选择微卫星标记，检测与该疾病抗性相关的QTL。同一连锁群上的免疫相关基因与显著的抗病QTL相关。特别是一些研究报道，qtl位于与MHC I类、II类或toll样受体区域相同的连锁群上。这些研究特别针对QTL区域的连锁群，因为它们携带经典的MHC I类和II类基因，这些基因先前与病毒、细菌和寄生虫病的易感性差异有关。在RT-3、RT-16、RT-2、RT-29、RT-27、RT-31、rt -17和RT-22连锁组中发现了一些与抗病相关的位点。这些QTL区域是抵抗链球菌感染的候选区域。