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Studies on the transcriptional regulation of fructan exohydrolase genes during regrowth after cutting in forage grasses

牧草砍伐后再生过程中果聚糖外切水解酶基因转录调控的研究

基本信息

批准号：
22780244
负责人：
TAMURA Ken-ichi
金额：
$ 2.5万
依托单位：
National Agriculture and Food Research Organization
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

项目摘要

A fructan exohydrolase gene, Pp6-FEH1 was transcriptionally induced in basal parts of timothy after cutting. This suggests that Pp6-FEH1 is involved in the energy supply for regrowth after cutting. Glucose and sucrose inhibited the up-regulation of Pp6-FEH1 transcripts. Transcriptional induction of Pp6-FEH1 by cold also suggests its involvement in increase of freezing tolerance by producing mono-saccharides known as cryoprotectants. Reporter assay revealed the promoter region involved in the cold induction of timothy FEH.

果聚糖外切水解酶基因Pp 6-FEH 1在梯牧草的基部被转录诱导。这表明Pp 6-FEH 1参与了切割后再生的能量供应。葡萄糖和蔗糖抑制Pp 6-FEH 1转录的上调。Pp 6-FEH 1的转录诱导冷也表明其参与通过产生称为冷冻保护剂的单核苷酸来增加冷冻耐受性。报告基因分析揭示了Timothy FEH冷诱导的启动子区域。