Function analysis of Eph/ephrin signals in angiogenesis

Eph/ephrin信号在血管生成中的功能分析

• 批准号: 22780262
• 负责人: ISHII Maki
• 金额: $ 2.25万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780262/
• 关键词: Eph; ephrin; 血管新生; マクロファージ; 血管内皮細胞; Eph/ephrin; レンチウイルスベクター; short hairpin RNA

In this study, it is intended to determine the function of Eph/ephrin in leucocyte-adhesion and inversion in angiogenesis. To require the data, preliminary experiments and functional analyses were conducted. It was determined that Eph/ephrin were expressed in blood vessels, and they adhered to leukocyte/macrophage and endothelial cells with cell processes contained high density actin fiber. These results indicate that the intercellular migration pathway for blood cells is formed by the pseudopodia of blood cells and the dilation of intercellular space by the membrane retraction in endothelial cells.

本研究旨在探讨Eph/ephrin在白细胞粘附和血管生成逆转中的作用。为了需要的数据，进行了初步的实验和功能分析。结果表明，Eph/ephrin在血管中表达，并与白细胞/巨噬细胞和内皮细胞粘附，细胞突起含有高密度的肌动蛋白纤维。这些结果表明，血细胞的伪足和内皮细胞膜收缩引起的细胞间隙扩张形成了血细胞的细胞间迁移途径。

项目成果

単球/マクロファージと血管内皮細胞に発現するEphA2とephrin-A1が接着に及ぼす影響

单核/巨噬细胞和血管内皮细胞表达的EphA2和ephrin-A1对粘附的影响

• 发表时间: 2011
• 作者: 佐伯法学;石井万幾;中島崇行;小川和重
• 通讯作者: 小川和重

心筋細胞におけるEphBの活性化とギャップ結合型情報伝達の阻害

EphB 激活和抑制心肌细胞间隙连接信号

• 发表时间: 2010
• 作者: ミュラー樹;石井万幾;中島崇行;小川和重
• 通讯作者: 小川和重

EphB signaling inhibits gap junctional intercellular communication and synchronized contraction in cultured cardiomyocytes

• DOI: 10.1007/s00395-011-0219-3
• 发表时间: 2011-11-01
• 期刊: BASIC RESEARCH IN CARDIOLOGY
• 影响因子: 9.5
• 作者: Ishii, Maki;Mueller, Itsuki;Ogawa, Kazushige
• 通讯作者: Ogawa, Kazushige

Effective and steady differentiation of a clonal derivate of P19CL6 embryonal carcinoma cell line into beating cardiomyocytes

P19CL6胚胎癌细胞系克隆衍生物有效稳定分化为跳动的心肌细胞

• 发表时间: 2010
• 期刊: J Biomed Biotechnol
• 作者: Mueller I;Kobayashi R;Nakajima T;Ishii M;Ogawa K
• 通讯作者: Ogawa K

Maeda N Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization

Maeda N 硫酸软骨素和硫酸乙酰肝素在早期神经元极化过程中的相反功能

• 发表时间: 2010
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Nishimura K;Ishii M;Kuraoka M;Kamimura K
• 通讯作者: Kamimura K