Study on molecular mechanisms of trypanosome cell differentiation for developing novel preventives against the parasite

研究锥虫细胞分化的分子机制以开发新型寄生虫预防剂

• 批准号: 22780269
• 负责人: SAKURAI Tatsuya
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780269/
• 关键词: 感染症; 原虫; Trypanosoma congolense; 細胞分化; 2D-DIGE

The cell differentiation from metacyclic form to bloodstream form of Trypanosoma congolense was induced in vitro, and the alternation of protein expression during the differentiation was analyzed by 2D-DIGE. The results showed that expressions of 15 proteins were increased but those of 9 proteins were decreased more than one and a half times after 6-hour induction of the differentiation. Meanwhile, expressions of 14 proteins were increased but those of 78 proteins were decreased more than twice after 12-hour induction of the differentiation.

在体外诱导了从元环状形式到血液形式的细胞分化，并通过2D-DIGE分析分化过程中蛋白质表达的交替。结果表明，15种蛋白质的表达增加了，但在6小时诱导分化后，9种蛋白质的表达降低了一半以上。同时，增加了14种蛋白质的表达，但在分化12小时后，78种蛋白质的表达降低了两次以上。

项目成果

Differential protein expression throughout lifecycle of Trypanosoma congolense, a major parasite of cattle in Africa

刚果锥虫（非洲牛的主要寄生虫）整个生命周期的差异蛋白表达

• 发表时间: 2011
• 期刊: Molecular and Biochemical Parasitology
• 影响因子: 1.5
• 作者: Hara K;Yoshdia S;Brett A.Eyford
• 通讯作者: Brett A.Eyford

アフリカトリパノソーマ症-獣医寄生虫学の挑戦-

非洲锥虫病——兽医寄生虫学的挑战——

• 发表时间: 2011
• 期刊: 北海道獣医師会雑誌
• 作者: 櫻井達也;井上昇
• 通讯作者: 井上昇

Identification and analyses of stage-specific surface protein toward revealing of trypanosome-tsetse interaction

鉴定和分析阶段特异性表面蛋白以揭示锥虫-采采蝇相互作用

• 发表时间: 2010
• 作者: Hara K;Yoshida S;Maria Teresa Armua-Fernandez;Kenshiro Hara;櫻井達也
• 通讯作者: 櫻井達也

Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids

• DOI: 10.1016/j.parint.2010.11.005
• 发表时间: 2011-01-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Armua-Fernandez, Maria Teresa;Nonaka, Nariaki;Oku, Yuzaburo
• 通讯作者: Oku, Yuzaburo

Structural characterization and epitope mapping of the Glutamic Acid/Alanine Rich protein from Trypanosoma congolense : Defining assembly on the parasite cell surface

刚果锥虫富含谷氨酸/丙氨酸的蛋白质的结构表征和表位作图：定义寄生虫细胞表面的组装

• DOI: 10.1074/jbc.m111.218941
• 发表时间: 2011
• 期刊: The Journal of Biological Chemistry
• 作者: Loveless;B. C.;Mason;J.W.;Sakurai;T.;Inoue;N.;Razavi;M.;Pearson;T. W. and Boulanger;M. J.
• 通讯作者: M. J.