Study on molecular mechanisms of trypanosome cell differentiation for developing novel preventives against the parasite

体外诱导刚果锥虫细胞从亚循环型向血流型分化，并利用二维DIGE技术分析其分化过程中蛋白质表达的变化。结果表明，诱导分化6h后，有15种蛋白表达量增加，但有9种蛋白表达量下降1.5倍以上。诱导分化12 h后，14种蛋白表达量增加，78种蛋白表达量减少2倍以上。

The cell differentiation from metacyclic form to bloodstream form of Trypanosoma congolense was induced in vitro, and the alternation of protein expression during the differentiation was analyzed by 2D-DIGE. The results showed that expressions of 15 proteins were increased but those of 9 proteins were decreased more than one and a half times after 6-hour induction of the differentiation. Meanwhile, expressions of 14 proteins were increased but those of 78 proteins were decreased more than twice after 12-hour induction of the differentiation.