Study on molecular mechanisms of trypanosome cell differentiation for developing novel preventives against the parasite

研究锥虫细胞分化的分子机制以开发新型寄生虫预防剂

• 批准号: 22780269
• 负责人: SAKURAI Tatsuya
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780269/
• 关键词: 感染症; 原虫; Trypanosoma congolense; 細胞分化; 2D-DIGE

The cell differentiation from metacyclic form to bloodstream form of Trypanosoma congolense was induced in vitro, and the alternation of protein expression during the differentiation was analyzed by 2D-DIGE. The results showed that expressions of 15 proteins were increased but those of 9 proteins were decreased more than one and a half times after 6-hour induction of the differentiation. Meanwhile, expressions of 14 proteins were increased but those of 78 proteins were decreased more than twice after 12-hour induction of the differentiation.

体外诱导刚果锥虫细胞从亚循环型向血流型分化，并利用二维DIGE技术分析其分化过程中蛋白质表达的变化。结果表明，诱导分化6h后，有15种蛋白表达量增加，但有9种蛋白表达量下降1.5倍以上。同时，14个蛋白质的表达量增加，但78个蛋白质的表达量减少了一倍以上的诱导分化12小时。

项目成果

Differential protein expression throughout lifecycle of Trypanosoma congolense, a major parasite of cattle in Africa

刚果锥虫（非洲牛的主要寄生虫）整个生命周期的差异蛋白表达

• 发表时间: 2011
• 期刊: Molecular and Biochemical Parasitology
• 影响因子: 1.5
• 作者: Hara K;Yoshdia S;Brett A.Eyford
• 通讯作者: Brett A.Eyford

アフリカトリパノソーマ症-獣医寄生虫学の挑戦-

非洲锥虫病——兽医寄生虫学的挑战——

• 发表时间: 2011
• 期刊: 北海道獣医師会雑誌
• 作者: 櫻井達也;井上昇
• 通讯作者: 井上昇

Identification and analyses of stage-specific surface protein toward revealing of trypanosome-tsetse interaction

鉴定和分析阶段特异性表面蛋白以揭示锥虫-采采蝇相互作用

• 发表时间: 2010
• 作者: Hara K;Yoshida S;Maria Teresa Armua-Fernandez;Kenshiro Hara;櫻井達也
• 通讯作者: 櫻井達也

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic.

利什曼病流行的秘鲁安第斯山脉沙蝇物种的基因分型。

• 发表时间: 2012
• 期刊: Acta Tropica
• 影响因子: 2.7
• 作者: M. Fujita;H. Kato;A. G. Cáceres;E. Gómez;L. Velez;T. Mimori;Feifei Zhang;H. Iwata;M. Korenaga;T. Sakurai;K. Katakura;Y. Hashiguchi
• 通讯作者: Y. Hashiguchi

Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids

• DOI: 10.1016/j.parint.2010.11.005
• 发表时间: 2011-01-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Armua-Fernandez, Maria Teresa;Nonaka, Nariaki;Oku, Yuzaburo
• 通讯作者: Oku, Yuzaburo