Establishment of the new molecular therapy for refractory canine bladder transitional cell carcinoma expressed HER-2.

表达 HER-2 的难治性犬膀胱移行细胞癌新分子疗法的建立。

• 批准号: 22780280
• 负责人: HOSHINO Yuki
• 金额: $ 2.58万
• 依托单位: Hokkaido University (2011-2013)Gifu University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780280/
• 关键词: 移行上皮癌; 分子標的治療; HER-2; 犬; 犬膀胱移行上皮癌; 膀胱移行上皮癌; 分子標的

We found that HER-2 overexpressed at the rate of 100% for canine bladder transitional cell carcinomas. Therefore we carried out research using molecular target therapy that targeted HER-2 as a new treatment for canine transitional cell carcinoma. Primary culturing of the transitional cell carcinoma obtained from canine cases was performed, and property analysis of the cultured cells was performed. As a result, three transitional cell carcinoma cell lines were established. The appearance of gene and protein expression of HER-2 was found for all three cell lines. However, as a result of the anticancer agent sensitivity examination, the anti-HER-2 agent, trastuzumab did not show cell cytotoxicity for transitional cell carcinomas. In vitro evaluation suggested that one of the antitumor effects of trastuzumab for canine transitional cell carcinomas requires an immune system response. Further study is needed to explore this response.

我们发现HER-2在犬膀胱移行细胞癌中呈100%的过度表达。因此，我们开展了以HER-2为靶点的分子靶向治疗犬移行细胞癌的研究。对犬移行细胞癌进行原代培养，并对培养的细胞进行特性分析。结果建立了3株移行细胞癌细胞系。3株细胞均出现HER-2基因和蛋白表达。然而，抗癌药物敏感性检测的结果是，抗HER-2的曲妥珠单抗对移行细胞癌没有显示细胞毒性。体外评估表明，曲妥珠单抗对犬移行细胞癌的抗肿瘤作用之一需要免疫系统反应。需要进一步的研究来探索这种反应。

项目成果

分子標的治療を目指した犬膀胱移行上皮癌細胞株の樹立

犬膀胱移行细胞癌细胞分子靶向治疗细胞系的建立

• 发表时间: 2012
• 作者: 田中知己;Jahid Zabuli;春名聡子;加茂前秀夫;星野有希,森崇,西谷由莉,酒井洋樹,奥村正裕,丸尾幸嗣
• 通讯作者: 星野有希,森崇,西谷由莉,酒井洋樹,奥村正裕,丸尾幸嗣

分標的治療を目指した犬膀胱移行上皮癌細胞株の樹立

犬膀胱移行细胞癌细胞靶向治疗细胞系的建立

• 发表时间: 2012
• 作者: 星野有希;他
• 通讯作者: 他

分子標的治療を目指した犬移行上皮癌細胞株の樹立

分子靶向治疗犬移行细胞癌细胞系的建立

• 作者: 高野絢子;大森優紀;好井健太郎;横澤香菜;苅和宏明;高島郁夫;楠本晃子,朝倉宏,Esho Firew Kassa,川本恵子;星野有希
• 通讯作者: 星野有希