Establishment of in vitro selection system using recent primary isolates, and a new efficient treatment for HIV

使用抗HIV-1药物进行体外筛选，以获得遗传异质性的HIV-1原代分离株。在体外筛选过程中，Env的降解率显著降低。此外，这些药物选择的变体具有与传代对照中完全不同的Env序列。这项研究是第一个探索的影响，抗艾滋病病毒药物的遗传瓶颈散装艾滋病病毒原代分离株含有高度多样化的Env序列，使用体外选择方法。体外选择可能是一个有用的工具，调查的兼容性联合抗逆转录病毒治疗使用进入抑制剂。

In vitro selection with anti-HIV-1 drugs were used to obtain genetically heterogeneous primary HIV-1 isolates. Env diversities significantly decreased during in vitro selection. Furthermore, these drugs-selected variants had completely different Env sequences from that in the passage control. This study is the first to explore the influence of anti-HIV drugs on genetic bottlenecks in bulk HIV primary isolates containing highly diverse Env sequences using in vitro selection methods. In vitro selection may be a useful tool for investigating the compatibility of combined anti-retroviral therapy using entry inhibitors.