Regulation of brain aging by renewal of microglia and recruitment of neural stem cell by intra-bone marrow-bone marrow transplantation

同基因骨髓间骨髓移植（IBM-BMT）后，骨髓来源的细胞迅速出现在缺乏血脑屏障（BBB）的软脑膜、脉络丛、血管周围区和心室周围器官中。骨髓来源的细胞进入了脑实质的几个小的离散区域，但它们没有进入其余的主要部分。骨髓来源的细胞表现出分叉的形态并表达 Iba-1，但不表达 GFAP、CNPase 或 NeuN，表明骨髓谱系。在以 SAMP10 小鼠为受体的嵌合体中，与以 B6 小鼠为受体的嵌合体相比，更多数量的供体来源的细胞进入更多的区域。同种异体IBM-BMT后，老SAMP10小鼠的学习能力没有改善，这可能是由于IBM-BMT后外周T细胞恢复失败所致。

After syngeneic intra-bone marrow-bone marrow transplantation(IBM-BMT), marrow-derived cells rapidly appeared in the leptomeninges, choroid plexus, perivascular regions and circumventricular organs, which lack the blood-brain barrier(BBB). Marrow-derived cells entered several small discrete regions of the brain parenchyma, although they did not enter the remaining major part. Marrow-derived cells exhibited ramified morphology and expressed Iba-1 but not GFAP, CNPase or NeuN, indicative of the myeloid lineage. In chimeras in which SAMP10 mice were recipients, a larger number of donor-derived cells entered more regions than in chimeras in which B6 mice were recipients. After allogeneic IBM-BMT, the learning ability of old SAMP10 mice was not ameliorated, which may be due to the failure in the recovery of peripheral T cells after IBM-BMT.