Regulation of brain aging by renewal of microglia and recruitment of neural stem cell by intra-bone marrow-bone marrow transplantation

通过骨髓内骨髓移植更新小胶质细胞和招募神经干细胞来调节脑衰老

• 批准号: 22790392
• 负责人: ISHII Sanae
• 金额: $ 2.5万
• 依托单位: Institute for Developmental Research, Aichi Human Service Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790392/
• 关键词: 骨髄内移植; ミクログリア; 脳老化病態; 骨髄内骨髄移植; 骨髄由来細胞; 脈絡叢; SAMP10マウス

After syngeneic intra-bone marrow-bone marrow transplantation(IBM-BMT), marrow-derived cells rapidly appeared in the leptomeninges, choroid plexus, perivascular regions and circumventricular organs, which lack the blood-brain barrier(BBB). Marrow-derived cells entered several small discrete regions of the brain parenchyma, although they did not enter the remaining major part. Marrow-derived cells exhibited ramified morphology and expressed Iba-1 but not GFAP, CNPase or NeuN, indicative of the myeloid lineage. In chimeras in which SAMP10 mice were recipients, a larger number of donor-derived cells entered more regions than in chimeras in which B6 mice were recipients. After allogeneic IBM-BMT, the learning ability of old SAMP10 mice was not ameliorated, which may be due to the failure in the recovery of peripheral T cells after IBM-BMT.

同基因骨髓内-骨髓移植（IBM-BMT）后，骨髓源性细胞迅速出现在缺乏血脑屏障（BBB）的软脑膜、脉络丛、血管周围区和脑室周围器官。骨髓来源的细胞进入脑实质的几个小的离散区域，尽管它们没有进入其余的主要部分。骨髓来源的细胞表现出分叉的形态，并表达Iba-1，但不表达GFAP，CNE 3或NeuN，表明髓系。在SAMP 10小鼠为受体的嵌合体中，与B6小鼠为受体的嵌合体相比，更多数量的供体来源细胞进入更多区域。异基因IBM-BMT后，老年SAMP 10小鼠的学习能力没有改善，这可能是由于IBM-BMT后外周血T细胞的恢复失败。

项目成果

Defects in cytokine-mediated neuroprotective glial responses to excitotoxic hippocampal injury in senescence-accelerated mouse

• DOI: 10.1016/j.bbi.2010.08.006
• 发表时间: 2011-01-01
• 期刊: BRAIN BEHAVIOR AND IMMUNITY
• 影响因子: 15.1
• 作者: Hasegawa-Ishii, Sanae;Takei, Shiro;Shimada, Atsuyoshi
• 通讯作者: Shimada, Atsuyoshi

The senescence-accelerated mouse (SAM) : Achievements and future directions

加速衰老小鼠（SAM）：成就和未来方向

• 发表时间: 2013
• 作者: ChenH;Shoumura S
• 通讯作者: Shoumura S

Amelioration of 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice by immunoregulatory dendritic cells

免疫调节树突状细胞改善2,4,6-三硝基苯磺酸诱导的小鼠结肠炎

• 发表时间: 2011
• 期刊: J.Gastroenterol.
• 作者: Hoshino S.;Kurishima A.;Inaba M.;Ando Y.;Fukui T.;Uchida K.;Nishio A.;Iwai H.;Yokoi T.;Ito T.;Hasegawa-Ishii S.;Shimada A.;M.Li;K.Okazaki;S.Ikehara
• 通讯作者: S.Ikehara

Proteomic analysis of aging brain in SAMP10 mouse: a model of age-related cerebral degeneration.

SAMP10 小鼠衰老大脑的蛋白质组学分析：年龄相关性脑退化模型。

• 发表时间: 2010
• 期刊: Mechanical Ageing and Develpoment 131
• 作者: Furukawa A;Oikawa S;Hasegawa-Ishii S;Chiba Y;Kawamura N;Takei S;Yoshikawa K;Hosokawa M;Kawanishi S;Shimada A
• 通讯作者: Shimada A

SAMP10に見られるサイトカインを介した神経保護的グリア応答の低下

SAMP10 中细胞因子介导的神经保护性神经胶质反应减少

• 发表时间: 2011
• 作者: 石井さなえ;稲葉宗夫;武井史郎;河村則子;千葉陽一;細川昌則;島田厚良
• 通讯作者: 島田厚良