Analysis of the phosphatydilinositol mediated lipid signal which involved in phagocytosis of the enteric protozoan parasite Entamoeba histolytica

参与肠道原生动物寄生虫溶组织内阿米巴吞噬作用的磷脂酰肌醇介导的脂质信号分析

• 批准号: 22790399
• 负责人: TSUKUI Kumiko
• 金额: $ 2.5万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790399/
• 关键词: 原虫; リソソーム; 赤痢アメーバ; 貪食; 加水分解酵素; 感染症; レセプター; 小胞輸送; イノシトールリン脂質; RhoGEF; Rac; PtdIns4P

In our previous study, we found the important role of phosphatidylinositol 3-phosphate during phagocytosis and phagosome maturation in enteric protozoan parasite Entamoeba histolytica. In this study we try to figure out the signaling from lipid mediator on phagosome maturation. As a result, we found a novel receptor molecule which involved in transport hydrolytic enzymes from endoplasmic reticulum to lysosomes or phagosomes and characterize their function. Cystene protease binding protein family 1(CPBF1) is identified as a binding protein of cysteine protease and there are 11 familial genes encorded in E. histolytica genome. At least 4 of them are bind to lysosomal hydrolase and we characterize CPBF8 in this study. It is suggests that these family molecules share their ligands and involved in lysosomal trafficking. In yeast or mammals there are receptors involved in ER to lysosome transport, i. e. MPR, VPS10p/Sortilin. CPBFs are functional homologue of these molecules but this is the first example the familial molecules involved in this process.

在我们以前的研究中，我们发现磷脂酰肌醇3-磷酸在肠道原虫溶组织内阿米巴的吞噬作用和吞噬体成熟过程中的重要作用。在本研究中，我们试图弄清楚脂质介质对吞噬体成熟的信号转导。因此，我们发现了一种新的受体分子，它参与将水解酶从内质网转运到溶酶体或吞噬体，并对其功能进行了表征。半胱氨酸蛋白酶结合蛋白家族1（Cystene protease binding protein family 1，CPBF 1）是半胱氨酸蛋白酶的结合蛋白，在大肠杆菌中编码11个家族基因。溶组织菌基因组其中至少有4个与溶酶体水解酶结合，我们在本研究中对CPBF 8进行了表征。这表明这些家族分子共享它们的配体并参与溶酶体运输。在酵母或哺乳动物中，存在参与ER向溶酶体转运的受体，即。e. MPR、VPS 10 p/分拣蛋白。CPBFs是这些分子的功能同源物，但这是参与该过程的家族分子的第一个例子。

项目成果

Localization and targeting of unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica.

溶组织内阿米巴中异常吡啶核苷酸转氢酶的定位和靶向。

• 发表时间: 2010
• 期刊: Eukaryot.Cell 9
• 作者: Yousuf M.A.;Mi-ichi F.;Nakada-Tsukui K.;Nozaki T.
• 通讯作者: Nozaki T.

赤痢アメーバの貪食の特異性に関与するRabの機能解析

Rab参与溶组织内阿米巴特异性吞噬作用的功能分析

• 发表时间: 2012
• 作者: 花舘有希;津久井久美子;野崎智義;中野由美子
• 通讯作者: 中野由美子

Identification and analysis of virulence-associated genes in Japanese clinical isolates

日本临床分离株毒力相关基因的鉴定与分析

• 发表时间: 2012
• 作者: Emi Sato;Kumiko Nakada-Tsukui;Aleyla Escueta;Makoto Kuroda;Takeshi Sekizuka;Tomoyoshi Nozaki
• 通讯作者: Tomoyoshi Nozaki

Trafficking mechanisms of phagosomal proteins in Entamoeba histolytica

溶组织内阿米巴吞噬体蛋白的运输机制

• 发表时间: 2011
• 作者: Atsushi Furukawa;Kumiko Tsukui;Tomoyoshi Nozaki.
• 通讯作者: Tomoyoshi Nozaki.

Phenotypic and transcriptional profiling in Entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance

• DOI: 10.3389/fmib.2015.00154
• 发表时间: 2015-05-05
• 期刊: FRONTIERS IN MICROBIOLOGY
• 影响因子: 5.2
• 作者: Penuliar, Gil M.;Nakada-Tsukui, Kumiko;Nozaki, Tomoyoshi
• 通讯作者: Nozaki, Tomoyoshi