Development of the methods for site-specific integration of LV vector with novel ZFP-LEDGF fusion protein

慢病毒载体基因组整合是基因表达必不可少的一步，但在宿主基因组中也发现了伴随这一步骤的突变。本项目的目的是发展定位整合LV载体的方法。我融合了整合LV载体所必需的蛋白质和DNA结合域，该结合域被设计用来结合序列特异性DNA。我找不到LV载体与这些融合蛋白的序列特异性整合。

Although lentiviral(LV) vector genomic integration step is essential for gene expression, mutations are found in host genome accompanied with this step. This aim of project is to develop of the methods for site-specific integration of LV vector. I fused the essential protein for integration of LV vector and DNA binding domain which was designed to bind sequence specific DNA. I could not find the sequence-specific integration of LV vector with these fusion proteins.