Development of the methods for site-specific integration of LV vector with novel ZFP-LEDGF fusion protein

虽然慢病毒（LV）载体基因组整合是基因表达的必要步骤，但在宿主基因组中发现了与此步骤相伴随的突变。这个项目的目的是开发LV向量在特定地点的整合方法。我融合了整合LV载体所需的蛋白质和DNA结合域，该结合域设计用于结合序列特异性DNA。我没有找到LV载体与这些融合蛋白的序列特异性整合。

Although lentiviral(LV) vector genomic integration step is essential for gene expression, mutations are found in host genome accompanied with this step. This aim of project is to develop of the methods for site-specific integration of LV vector. I fused the essential protein for integration of LV vector and DNA binding domain which was designed to bind sequence specific DNA. I could not find the sequence-specific integration of LV vector with these fusion proteins.