Causal relationship among the exhaled gas constituents, oxidative stress markers and smoking behaviors in smokers

建立了气相色谱/质谱联用和液相色谱/质谱联用的呼气末挥发性有机物、尿8-异前列腺素和尼古丁代谢物的固相萃取测定方法。统计分析其与氧化应激标志物、尼古丁代谢物及吸烟方式的关系。尿中8-OHdG含量与每日吸烟数、每日吸烟总容积、尿可替宁含量差异有统计学意义（p<0.05）。iPF_<2α->III在年龄、唾液可替宁、尿液3-羟基可替宁中含量差异均有统计学意义（p<0.05）。另一方面，没有观察到与呼吸一氧化碳和氧化应激标志物的关联。

We established the methods for determination of the terminal-expiratory volatile organic compounds, urinary 8-isoprostane and nicotine metabolites by solid phase extraction with GC/MS and LC/MS/MS. The association with the oxidation stress marker, nicotine metabolites and the smoking pattern about smokers was statistically analyzed. The amounts of urinary 8-OHdG was statistically significant in number of cigarettes smoked per day, total smoked puff volume per day and urinary cotinine (p<0.05). The amounts of iPF_<2α->III was statistically significant in the age, salivary cotinine and urinary 3-hydroxycotinine (p<0.05). On the other hand, the association with breath carbon monoxide and oxidative stress markers was not observed.