Estimation of the time of death by clock gene mRNA expression

我们假设昼夜节律相关基因mRNA表达水平与死亡时间相关，以期在法医领域建立新的分子生物学估计方法。选择Arntl、Clock、Cry和Per2作为靶基因，对每隔6小时处死的小鼠进行实时pcr分析。一些在脑或肾中表现出动态表达变化的基因似乎有助于估计死亡时间。

We hypothesized that circadian rhythm-related gene mRNA expression level correlates to the time of death in order to establish the new estimation method using molecular biological techniques in forensic field. Arntl, Clock, Cry, and Per2 were chosen as target genes for realtime-PCR analysis in mice sacrificed every 6 hrs interval. Some genes showing dynamic expression change in Brain or Kidney seemed to be useful to estimate the time of death.