Establishment of hepatic stellate cell de-activation in liver fibrosis model using 3 dimensional bio artificial liver

将永生化的小鼠星状细胞、内皮细胞和肝细胞在径向流生物反应器中共培养的生物人工肝模型在常规培养液（2%FBS +ASF-104 N）中显示肝纤维化活性。当在该生物人工肝模型中加入抗纤维化药物时，反映将维生素A储存到星状细胞中的能力的LRAT在mRNA和蛋白水平上重新表达。

Bio artificial liver model which is co-cultured with immortal mouse stellate cell, endothelial cell and hepatocyte using radial flow bioreactor showed activity of liver fibrosis in conventional culture fluid(2% FBS+ASF-104N). When an anti-fibrotic agent was added in this bio artificial liver model, LRAT which reflect ability to store vitamin A into stellate cell was re-expressed in mRNA and protein level.