Evaluating Plasma Endothelial Cell-derived Micro particles for Detecting Abnormal Expression of Molecules Involved in Onset of Pulmonary Arterial Hypertension Associated with Connective Tissue Diseases

评估血浆内皮细胞来源的微粒以检测与结缔组织疾病相关的肺动脉高压发作相关分子的异常表达

• 批准号: 22790687
• 负责人: MARUYAMA Hidekazu
• 金额: $ 2.75万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790687/
• 关键词: 肺高血圧症; 血管内皮細胞; バイオマー Jー; マイクロパーティクル; 膜タンパク; 膠原病; 接触阻害; セロトニン; バイオマーカー; miRNA

We aimed to establish a new method for diagnosing pulmonary arterial hypertension. The endothelial cell-derived microparteicles in plasma are quantified targeting six surface antigens, although, we could not find significant change between the patients and controls. To select new surface markers, we created an in vitro model using cultivated endothelial cells evaluated by impairment of contact inhibition. Certain two factors, which are thought to be related to onset of the disease, were essential for this impairment.

本研究旨在建立一种诊断肺动脉高压的新方法。血浆中内皮细胞衍生的微粒针对六种表面抗原进行定量，尽管我们没有发现患者和对照组之间的显著变化。为了选择新的表面标志物，我们使用培养的内皮细胞建立了一个体外模型，通过接触抑制的损害进行评价。有两个因素被认为与疾病的发生有关，对这种损害至关重要。