Evaluating Plasma Endothelial Cell-derived Micro particles for Detecting Abnormal Expression of Molecules Involved in Onset of Pulmonary Arterial Hypertension Associated with Connective Tissue Diseases

我们的目的是建立一种诊断肺动脉高压的新方法。针对6种表面抗原，我们对血浆中内皮细胞来源的微颗粒进行了定量，但在患者和对照组之间没有发现明显的变化。为了选择新的表面标记，我们创建了一个使用培养的内皮细胞的体外模型，通过接触抑制的损害来评估。某些被认为与疾病发病有关的两个因素对这种损害是必不可少的。

We aimed to establish a new method for diagnosing pulmonary arterial hypertension. The endothelial cell-derived microparteicles in plasma are quantified targeting six surface antigens, although, we could not find significant change between the patients and controls. To select new surface markers, we created an in vitro model using cultivated endothelial cells evaluated by impairment of contact inhibition. Certain two factors, which are thought to be related to onset of the disease, were essential for this impairment.