Evaluating Plasma Endothelial Cell-derived Micro particles for Detecting Abnormal Expression of Molecules Involved in Onset of Pulmonary Arterial Hypertension Associated with Connective Tissue Diseases

我们旨在建立一种诊断肺动脉高压的新方法。血浆中内皮细胞来源的微颗粒针对六种表面抗原进行了定量，尽管我们没有发现患者和对照组之间的显著变化。为了选择新的表面标记，我们用培养的内皮细胞建立了一个体外模型，通过评估接触抑制的损伤。有两个因素被认为与这种疾病的发病有关，它们对这种损害是必不可少的。

We aimed to establish a new method for diagnosing pulmonary arterial hypertension. The endothelial cell-derived microparteicles in plasma are quantified targeting six surface antigens, although, we could not find significant change between the patients and controls. To select new surface markers, we created an in vitro model using cultivated endothelial cells evaluated by impairment of contact inhibition. Certain two factors, which are thought to be related to onset of the disease, were essential for this impairment.