Functional analysis of cytokeratin-8 on invasion and metastasis of lung cancer cell lines

为了探讨ck在肺癌细胞侵袭中的作用，我们采用定量免疫印迹法研究了ck在非小细胞肺癌（NSCLC）细胞系中的表达水平。HI1017表达CK8和18；A549分别表达CK8、18和19。利用Matrigel系统对有创细胞进行反复筛选，建立有创亚系，与亲本细胞相比，其表达水平较低。外源性CK19也导致HI1017侵袭性降低。通过短干扰rna抑制CK8或CK18导致总ck减少和两种细胞系的侵袭性增加。A549表达极低水平的CK19。抑制CK19既不影响A549的侵袭能力，也不影响CK总量。我们的观察结果表明，CK表达水平与NSCLC细胞系的侵袭性呈负相关，表明显性CK的表达水平可能影响侵袭能力。

In oder to investigate the role of CKs in the invasion of lung cancer cells, we investigated the expression levels of CKs in non-small cell lung cancer (NSCLC) cell lines by quantitative immunoblotting. HI1017 expressed CK8 and 18; A549 expressed CK8, 18 and 19, respectively. Invasive sublines were established by repeated selection of invasive cells using Matrigel system and showed lower expression levels of CKs compared with the parental cells. Exogenous CK19 also resulted in a decrease in invasiveness of the HI1017. Suppression of either CK8 or CK18 by short interfering RNAs led to a decrease in the total CKs and increased invasiveness of both cell lines. A549 expressed very low levels of CK19. Suppression of CK19 affected neither invasive ability nor total CK amount in the A549. Our observations indicate that CK expression levels were inversely associated with invasiveness of the NSCLC cell lines, and suggest that expression levels of dominant CKs may affect invasive ability.