Directed differentiation of human iPS cells into nephrogenic mesoderm towards regenerative medicine strategy for kidney diseases

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We have succeeded in generating reporter human iPS cell lines for OSR1 gene, which is a specific marker for an embryonic germ layer, the intermediate mesoderm(IM) that gives rise to kidney. By using combinatorial treatment of growth factors, we have established highly efficient differentiation methods to induce human iPS cells into IM cells at an induction rate of more than 90%. We have also confirmed that these human IM cells can differentiate into cells constituting IM derivative organs, such as kidney, adrenal cortex and genitals.