Analysis of mechanism of albuminuria/proteinuria in diabetes in terms of sugar chain

从糖链角度分析糖尿病白蛋白尿/蛋白尿机制

• 批准号: 22790788
• 负责人: IWATANI Hirotsugu
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790788/
• 关键词: 蛋白尿; アルブミン尿; チャージバリア; 糖鎖; 糖尿病; 蛋白尿・アルブミン尿; 内皮細胞; たこ足細胞

Charge barrier and size barrier have been postulated as a glomerular filtration barrier. We found less staining of WGA lectin which recognizes sialic acid in glomeruli of STZ-induced diabetic mice by histochemical staining and immuno-blotting method, indicating the decrease of negative charge. However, comprehensive analysis of murine glomerular protein which decreased in its WGA-binding property by mass spectrometry to date did not reveal any candidate protein such as podocalyxin which has been supposed to be a carrier of negative charge.

电荷屏障和大小屏障被认为是肾小球滤过屏障。免疫组化染色和免疫印迹法发现糖尿病小鼠肾小球内识别唾液酸的WGA凝集素染色减少，表明负电荷减少。然而，迄今为止，通过质谱法对WGA结合特性降低的鼠肾小球蛋白进行的综合分析没有发现任何候选蛋白，例如被认为是负电荷载体的足糖萼蛋白。

项目成果

ストレス下における腎構成細胞の糖鎖発現について

关于应激下肾脏组成细胞的糖链表达

• 发表时间: 2010
• 作者: 川崎靖;横林恵理子;坂本健太郎;高木はるか;米澤正;杉山晶規;名取泰博;岩谷博次
• 通讯作者: 岩谷博次