Analysis of mechanism of albuminuria/proteinuria in diabetes in terms of sugar chain

电荷屏障和大小屏障被认为是肾小球滤过屏障。免疫组化染色和免疫印迹法发现糖尿病小鼠肾小球内识别唾液酸的WGA凝集素染色减少，表明负电荷减少。然而，迄今为止，通过质谱法对WGA结合特性降低的鼠肾小球蛋白进行的综合分析没有发现任何候选蛋白，例如被认为是负电荷载体的足糖萼蛋白。

Charge barrier and size barrier have been postulated as a glomerular filtration barrier. We found less staining of WGA lectin which recognizes sialic acid in glomeruli of STZ-induced diabetic mice by histochemical staining and immuno-blotting method, indicating the decrease of negative charge. However, comprehensive analysis of murine glomerular protein which decreased in its WGA-binding property by mass spectrometry to date did not reveal any candidate protein such as podocalyxin which has been supposed to be a carrier of negative charge.