Regulators for Ustilago maydis mig gene expression during pathogenic development

致病发育过程中玉米黑粉菌 mig 基因表达的调节因子

• 批准号: 5399155
• 负责人: Dr. Christoph Basse
• 金额: --
• 依托单位: Abteilung Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5399155?language=en
• 关键词: Regulators; Ustilago; maydis; mig; gene

The corn pathogen Ustilago maydis requires its host plant maize for development and completion of its sexual life cycle. We have identified the Ustilago maydis genes mig1 and mig2 that show a plant specific expression profile. The mig2 genes are organized in a cluster comprising five highly homologous genes, designated mig2-1 to mig2-5. In the promoters of the mig2-1 and mig2-5 genes distinct negatively- and positively cis-acting regions have been mapped. In particular, a highly conserved 40bp region has been identified in the mig2-5 promoter that is absolutely required for gene activation during the biotrophic growth phase. In this proposal we wish to delineate the respective consensus sequences and to isolate the transcriptional regulator(s) from U. maydis that bind(s) to this region by a biochemical approach based on gel retardation analysis and a yeast one-hybrid screen. The gene(s) encoding the binding activity will be analysed and U. maydis mutants deleted in identified mig2 activator genes will be generated to investigate their impact on mig gene regulation and pathogenicity. Furthermore, identified mig2 activator genes will be expressed under control of an inducible promoter to assess whether overexpression is sufficient to trigger mig gene expression during axenic growth. If successful, conditional activator strains will be exploited to screen for additional target genes by microarray analysis.

玉米黑粉菌（Ustilago maydis）需要其寄主植物玉米来发育和完成其性生活周期。我们已经确定了玉米黑粉菌基因mig 1和mig 2，显示植物特异性表达谱。mig 2基因被组织成包含五个高度同源基因的簇，命名为mig 2 -1至mig 2 -5。在mig 2 -1和mig 2 -5基因的启动子中，已经定位了不同的负顺式和正顺式作用区域。特别地，在mig 2 -5启动子中已经鉴定出高度保守的40 bp区域，其是在活体营养生长阶段期间基因激活所绝对需要的。在这个建议中，我们希望描绘各自的共有序列，并从U.玉米通过基于凝胶阻滞分析和酵母单杂交筛选的生化方法与该区域结合。将对编码结合活性的基因进行分析，并进行U.将产生在鉴定的mig 2激活基因中缺失的玉米突变体，以研究它们对mig基因调控和致病性的影响。此外，鉴定的mig 2激活基因将在诱导型启动子的控制下表达，以评估过表达是否足以在无菌生长期间触发mig基因表达。如果成功的话，将利用条件激活菌株通过微阵列分析筛选额外的靶基因。