Cardioprotective effect of intravascular mild hypothermia against ischemia/reperfusion injury in rat isolated heart model

血管内亚低温对大鼠离体心脏模型缺血/再灌注损伤的心脏保护作用

• 批准号: 22592012
• 负责人: MOCHIZUKI Toshiaki
• 金额: $ 2.16万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592012/
• 关键词: 救急蘇生学; 虚血再灌流障害; 心筋保護; 低体温; ERK; 心肺蘇生; infarct size; viability; ischemia/reperfusion injury; reactive oxygen species (ROS); cooling; nitric oxide (NO); Phosphoinositide 3-kinase (PI3K); reactive oxygen species

A cardioprotective effect was present when hypothermia at 34 ℃ was induced during ischemia, and this effect still occurred when induction of hypothermia was early after reperfusion. Futhermore, the cardioprotective effect of hypothermia, was not observed when 10 min of 15 mmHg low-reperfusion immediately after ischemia was added to reperfusion period, but did occur when 10 min of 40 mmHg low-reperfusion immediately after ischemia was added. The phosphoinositide 3'kinase(PI3K)/nitric oxide (NO) pathway, and extracellular signal-regulated kinase(ERK) pathway were involved in the mechanism underlying the effect of hypothermia.

缺血时34 ℃低温对心肌有保护作用，再灌注后早期低温对心肌仍有保护作用。再灌注期加15 mmHg低灌注10 min时，未观察到低温的心脏保护作用Fuximine，而加40 mmHg低灌注10 min时，则观察到Fuximine。磷脂酰肌醇3 '激酶（PI 3 K）/一氧化氮（NO）通路和细胞外信号调节激酶（ERK）通路参与了低温的作用机制。

项目成果

QUALITY OF CARDIOPULMONARY RESUSCITATION AFFECTS CARDIOPROTECTION BY INDUCED HYPOTHERMIA AT 34°C AGAINST ISCHEMIA/REPERFUSION INJURY IN A RAT ISOLATED HEART MODEL

• DOI: 10.1097/shk.0b013e318294e259
• 发表时间: 2013-06-01
• 期刊: SHOCK
• 影响因子: 3.1
• 作者: Mochizuki, Toshiaki;Jiang, Qiliang;Sato, Shigehito
• 通讯作者: Sato, Shigehito

低体温療法における心筋保護:基礎研究から臨床応用へ

低温治疗期间的心肌保护：从基础研究到临床应用

• 发表时间: 2013
• 作者: Osamu Sakata;Makoto Ohki;Yoichi Saito;望月利昭
• 通讯作者: 望月利昭

Extracellular Signal-regulated kinase は心肺蘇生をシミュレートした虚血再灌流モデルに対する34℃低体温療法の心筋保護作用を介する

细胞外信号调节激酶在模拟心肺复苏的缺血再灌注模型中介导34℃低温的心肌保护作用

• 发表时间: 2012
• 作者: 望月利昭;川島信吾;成瀬智;吉野篤人;土 井 松 幸 ; 佐 藤 重 仁
• 通讯作者: 佐 藤 重 仁

Mild hypothermia induced cardioprotection against ischemia/reperfusion injury mediated by nitric oxide in rat isolated heart

亚低温诱导大鼠离体心脏对一氧化氮介导的缺血/再灌注损伤的心脏保护作用

• 发表时间: 2011
• 作者: Mochizuki T.;Yu S.;Katoh T.;Aoki K.;Sato S.
• 通讯作者: Sato S.

心肺蘇生と脳保護 麻酔科学レビュー

心肺复苏和脑保护麻醉学综述

• 发表时间: 2013
• 作者: 望月利昭;余樹春;吉野篤人;青木克憲;佐藤重仁;蟇目将英;望月利昭
• 通讯作者: 望月利昭