tudy of therapeutic effect of sodium nitrite on crush syndrome

亚硝酸钠治疗挤压综合征的疗效研究

• 批准号: 22592021
• 负责人: KOBAYASHI Jun
• 金额: $ 2.33万
• 依托单位: Josai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592021/
• 关键词: クラッシュ症候群; 虚血再灌流; 一酸化窒素; 亜硝酸; デキサメサゾン; 虚血再灌漑

We developed crush syndrome (CS) rat model to evaluate the therapeutic effect of nitrite on crush syndrome. Intraperitoneal single injection of nitrite improved survival rate of this CS model from 25% to 64%, furthermore combined therapy of continuous infusion of nitrite with conventional therapies including massive fluid infusion and correction of acidosis resulted in 100% survival rate. This might be due to the tissue protective effect of nitrite-derived nitric oxide (NO) against ischemia/reperfusion injury. A 100% survival rate was also achieved by the treatment of the CS rat with single injection of dexamethasone through a mechanism involving NO production in the injured skeletal muscle．These results provide not only a new protective mechanism against ischemia/reperfusion injury in this syndrome, but also a simple and promising drug therapy especially useful in the field of disasters

本研究通过建立挤压综合征（CS）大鼠模型，探讨亚硝酸盐对挤压综合征的治疗作用。单次腹腔注射亚硝酸盐可使该CS模型的存活率从25%提高到64%，持续输注亚硝酸盐与常规治疗（包括大量补液和纠正酸中毒）的联合治疗可使该CS模型的存活率达到100%。这可能是由于亚硝酸盐衍生的一氧化氮（NO）对缺血/再灌注损伤的组织保护作用。单次注射地塞米松可使CS大鼠获得100%的存活率，这一结果不仅为CS大鼠的缺血再灌注损伤提供了一种新的保护机制，而且也为灾难性疾病的治疗提供了一种简便可行的药物治疗方法

项目成果

Nitrite ameliorates ischemia/reperfusion injury of skeletal muscle in rat crush injury model

亚硝酸盐改善大鼠挤压损伤模型中骨骼肌缺血/再灌注损伤

• 发表时间: 2011
• 作者: Jun Kobayashi;Ryo Nozaki;Isamu Murata;Kazuo Ohtake;Kazuya Ooi;Kunihiro Sonoda;Genya Nakano;Soichiro Kimura;Hideo Ueda;Hiroyuki Uchida;Yasunori Morimoto
• 通讯作者: Yasunori Morimoto

Nitrite reduces ischemia/reperfusion-induced muscle damage and improves survival rates in rat crush injury model

• DOI: 10.1097/ta.0b013e31824a76b5
• 发表时间: 2012-06-01
• 期刊: JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
• 影响因子: 3.4
• 作者: Murata, Isamu;Nozaki, Ryo;Kobayashi, Jun
• 通讯作者: Kobayashi, Jun