Experimental study of free muscle transplantation with microneurovascular anastomosis -Analysis of acetylcholine receptor in transplanted muscle-

微神经血管吻合术游离肌移植的实验研究-移植肌乙酰胆碱受体分析-

• 批准号: 22591999
• 负责人: KASHIWA Katsuhiko
• 金额: $ 2.5万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591999/
• 关键词: マイクロサージェリー学; 筋肉移植; 神経再生; アセチルコリン; レセプター; アセチルコリンレセプター; マイクロサージャリ-; 筋再生; αブンガロトキシン; 蛍光染色; マイクロサージャリー学

Experimental study using the rectus femoris muscle of rabbits was performed to investigate the recovery process of the muscle transplantation with microneurovascular anastomosis.After 2~24 postoperative weeks, tissue specimens obtained from muscle tissue were examined for pathological analysis. In the pathological analysis, fluorescent staining was used for visualizing the muscle fibers and distribution of acetylcholine receptors together with H-E staining and Azan staining.Wet weight of transplanted muscle had gradually increased after it decreased to about half of control model in innervation model. Degeneration and recovery of muscle fibers were confirmed by H-E and Azan staining in the each model, although the comfortable result for statistical analysis could not be obtained in the fluorescent staining, because the determination of the distribution of acetylcholine receptor was unstable.

采用兔股直肌为实验材料，观察吻合微神经血管的肌移植的恢复过程，术后2~24周取肌组织标本进行病理学分析。病理学分析采用荧光染色、H-E染色、Azan染色观察肌纤维及乙酰胆碱受体的分布，神经支配模型移植肌湿重下降至对照模型的一半左右后逐渐增加。在每个模型中，通过H-E和Azan染色证实了肌纤维的变性和恢复，尽管在荧光染色中不能获得用于统计分析的舒适结果，因为乙酰胆碱受体分布的测定不稳定。