Experimental study of free muscle transplantation with microneurovascular anastomosis -Analysis of acetylcholine receptor in transplanted muscle-

以兔股直肌为实验材料，观察吻合微神经血管的肌肉移植术后恢复过程，术后2~24周取肌组织标本进行病理学分析。病理分析采用荧光染色结合H-E染色和Azan染色显示肌纤维和乙酰胆碱受体的分布，在神经支配模型中，移植肌肉的湿重在减少到对照模型的一半后逐渐增加。H-E和Azan染色证实了各模型肌纤维的变性和恢复，但由于乙酰胆碱受体分布的测定不稳定，荧光染色不能获得令人满意的统计分析结果。

Experimental study using the rectus femoris muscle of rabbits was performed to investigate the recovery process of the muscle transplantation with microneurovascular anastomosis.After 2~24 postoperative weeks, tissue specimens obtained from muscle tissue were examined for pathological analysis. In the pathological analysis, fluorescent staining was used for visualizing the muscle fibers and distribution of acetylcholine receptors together with H-E staining and Azan staining.Wet weight of transplanted muscle had gradually increased after it decreased to about half of control model in innervation model. Degeneration and recovery of muscle fibers were confirmed by H-E and Azan staining in the each model, although the comfortable result for statistical analysis could not be obtained in the fluorescent staining, because the determination of the distribution of acetylcholine receptor was unstable.