Regulation of mast cells by extracelluar matrix using self-organizing nano-technology method

我们研究了蜂窝膜对肥大细胞增殖的调节作用。我们使用了两种类型的肥大细胞，NCL-2细胞(新的非肿瘤肥大细胞系)和RBL-2H3细胞(大鼠嗜碱性白血病细胞系)。在3、5和10微米的HCF上培养的细胞均表现出增殖率、细胞形成率和肥大细胞释放介质的变化，但HCF的质量相同。而HCF的调节依赖于一种细胞的差异。与人粘膜肥大细胞相似的NCL-2细胞在HCF上增殖，但可减少肥大细胞中从头合成的细胞因子--肿瘤坏死因子-α和白三烯B4的释放。

We studied mast cell proliferation and regulation by honeycomb film (HCF). We used two types of mast cells, NCL-2 cell (new non-tumor mast cell line) and RBL-2H3 cell (rat basophilic leukemia cell line). Both of the cells cultured on 3, 5, and 10 micro m pore size of HCF revealed changing of proliferation rate, cell formation, and mediator release from mast cell though the quality of HCF is same. And then HCF regulation depended on the difference of a kind of cell. NCL-2 cell that is similar to human mucosal mast cell proliferated on the HCF, but reduced the release of TNF-alpha and leukotriene B4, which are de novo synthesized cytokines in mast cell.