Involvement of excess neuropeptide by axon reflex in idiopathic pulpitis

本研究中，AEA（Anandamide）、CGRP（Calcitonin gene-related peptide）、VIP（Vasoactive intestinal peptide）和P物质诱导培养的HPC（human cells dental pulp cells）产生MMP-2。HPC表达所有3种类型的AEA受体（CB 1、CB 2和TRPV-1）。AEA诱导的MMP-2的产生被CB 1或TRPV-1拮抗剂和CB 1或TRPV-1的小干扰RNA阻断。此外，c-jun N-末端激酶抑制剂也减少MMP-2的产生。我们首次证明AEA通过CB 1和TRPV-1诱导HPC中MMP-2的产生。

In this study, AEA (Anandamide), CGRP (Calcitonin gene-related peptide), VIP (Vasoactive intestinal peptide) and SubstanceP induced MMP-2 production in HPC (human cells dental pulp cells) in culture. HPC expressed all 3 types of AEA receptor (CB1, CB2, and TRPV-1). AEA-induced MMP-2 production was blocked by CB1 or TRPV-1 antagonists and by small interfering RNA for CB1 or TRPV-1. Furthermore, c-jun N-termialkinase inhibitor also reduced MMP-2 production. We demonstrated for the first time that AEA induced MMP-2 production via CB1 and TRPV-1 in HPC.