Involvement of excess neuropeptide by axon reflex in idiopathic pulpitis

在本研究中，AEA （Anandamide）、CGRP（降钙素基因相关肽）、VIP （Vasoactive intestinal peptide）和substance ep诱导培养的HPC（人牙髓细胞）产生MMP-2。HPC表达了3种AEA受体（CB1、CB2和TRPV-1）。aea诱导的MMP-2生成可被CB1或TRPV-1拮抗剂以及CB1或TRPV-1的小干扰RNA阻断。此外，c-jun N-termialkinase inhibitor也能减少MMP-2的产生。我们首次证明了AEA在HPC中通过CB1和TRPV-1诱导MMP-2的产生。

In this study, AEA (Anandamide), CGRP (Calcitonin gene-related peptide), VIP (Vasoactive intestinal peptide) and SubstanceP induced MMP-2 production in HPC (human cells dental pulp cells) in culture. HPC expressed all 3 types of AEA receptor (CB1, CB2, and TRPV-1). AEA-induced MMP-2 production was blocked by CB1 or TRPV-1 antagonists and by small interfering RNA for CB1 or TRPV-1. Furthermore, c-jun N-termialkinase inhibitor also reduced MMP-2 production. We demonstrated for the first time that AEA induced MMP-2 production via CB1 and TRPV-1 in HPC.