Capability of Regeneration Therapy with Peripheral Mononuclear BloodCells(PBMCs)

在体外培养实验中，培养第3天的骨髓细胞数量增加，在PBMC中呈浓度依赖性地呈现较多的细胞数量。细胞代谢活性也随着PBMC浓度的增加而增加，最高可达40%。在放射学检查中，移植组早期即可观察到松质骨的形成，皮质骨的形成受到抑制。在组织学研究中，也观察到了类似的结果。移植组松质骨形成增加，随着PBMCs的加入，体内和体外成骨细胞的功能表型均增加。这些发现为进一步在体外和体内探索PBMCs作为骨再生细胞治疗的新来源提供了依据。

In vitro study, the number of cultured bone marrow cells cultured at day 3 increased in the PBMC concentration-dependently showed greater number of cells. Cell metabolic activity also increased with an increase of PBMC concentration up to by40%. Likewise, ALP activity increased at day 5 and 10 with PBMC.In radiological study, cancellous bone formation was observed from early time point and cortical bone formation was inhibited in the transplantation group. In histologicalstudy, similar results were observed. Increase of cancellous bone formation was observed in transplantation group.An addition of the PBMCs, elevated functional phenotypes of osteoprogenitor cells in vitro and in vivo. These findings warrants further in-vitro and in-vivo exploration of PBMCs as a novel source of cell therapy for bone regeneration.