Analysis of the early aberrant DNA methylation and influence of demethylation agent in the 4NQO-induced rat tongue cancer model

点击翻译按钮获取中文摘要

Epigenetic alteration attracts attention of the carcinogenesis process. However, it is difficult to examine at early time without the lesion. Therefore we examined in4NQO-induced rat tongue carcinogenesis model. The gene examined p16 and MGMT .In addition, we examined epigallocatechin gallate (EGCG) about demethylating ability .As a result, the significant change of the methylation status did not occur in each period. However, slightly controlled tendency was seen in the protein level in EGCGgroup. By these results, the carcinogenesis agent does not bring the epigenetic alteration, immediately . It was shown that long-term sustained influence participated.Furthermore, the possibility was shown that EGCG was effective by the long-term dosage.