MFCS4 in palate development

MFCS4 在味觉发育中的作用

• 批准号: 22592254
• 负责人: OKUHARA Shigeru
• 金额: $ 2.83万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592254/
• 关键词: 病態検査学; 口蓋裂; 口蓋; ソニック:ヘッジホッグ; ソニックヘッジホック; エンハンサー

MFCS4 is one of cis-regulatory sequence for Shh transcription and its activation was found in oral and pharyngeal region from E11.00-E15.50. Compound deletion heterozygotes for Shh and MFCS4 show cleft palate. In palatal development, elevation of palatal shelves is achieved by corporation of intrinsic elevation force and change in shape and motion of the tongue. Because the palate of the mutant without tongue in organ culture successfully elevated and the exterior and histological preparation of the mutant tongue was hypomorphic, we focused on the relationship between tongue development and Shh signaling. In the tongue of the mutant, arrangement of the myotube was not organized or symmetric. Basal membrane of lingual epithelium that was discontinuous and without wave, and lingual septum that was again discontinuous and hypomorphic, were suggested to cause myotube disarrangement. As Shh signaling was previously reported to regulate the expression of laminin, that is a key component for basal membrane, and Sox9, that is a master transcription factor for tendon development, histological significances found in the tongue of the mutant agree with molecular mechanisms. These findings will be published with molecular evidences.

MFCS 4是Shh转录的顺式调控序列之一，在E11.00-E15.50的口腔和咽部区域发现其激活。Shh和MFCS 4复合缺失杂合子显示腭裂。在腭发育过程中，腭架的提升是通过舌的内在提升力和舌的形状和运动的变化来实现的。由于无舌突变体的腭在器官培养中成功地升高，并且突变体舌的外观和组织学制备是亚形态的，因此我们专注于舌发育和Shh信号之间的关系。在突变体的舌头，肌管的安排是不组织或对称。舌上皮基底膜不连续且无波形，舌隔也不连续且亚形，这可能是导致肌管排列紊乱的原因。由于Shh信号先前被报道调节层粘连蛋白（其是基底膜的关键组分）和Sox 9（其是腱发育的主转录因子）的表达，在突变体的舌中发现的组织学意义与分子机制一致。这些发现将与分子证据一起发表。

项目成果

Tongue and palate development in Shh-/+MFCS4+/-

Shh-/ MFCS4 /- 舌头和上颚的发育

• 发表时间: 2012
• 作者: Shigeru OKUHARA;Ryosuke NAGAOKA;Takanori AMANO;Tomoko SAGAI;Toshihiko SHIROISHI;Sachiko ISEKI
• 通讯作者: Sachiko ISEKI

Cleft palate in compound heterozygote of sonic hedgehog and MFCS4

声波刺猬和 MFCS4 复合杂合子中的腭裂

• 发表时间: 2010
• 作者: Okuhara S.;Sagai T.;Nagaoka R.;Amano T.;Shiroishi T.;Iseki S.
• 通讯作者: Iseki S.

胎児期低酸素状態が顔面形成に与える影響

胎儿缺氧对面部形成的影响

• 发表时间: 2010
• 作者: 長岡亮介;奥原滋;天笠光雄;井関祥子
• 通讯作者: 井関祥子

Shh in development of pharyngeal region

咽部发育中的“嘘”

• 发表时间: 2012
• 作者: Shigeru OKUHARA;Ryosuke NAGAOKA,Takanori AMANO;Tomoko SAGAI;ToshihikoSHIROISHI;Sachiko ISEKI;田野智之;Shigeru Okuhara
• 通讯作者: Shigeru Okuhara

Tongue and palate development in Shh-/+MFCS4+/-.

舌头和上颚的发育处于Shh-/ MFCS4 /-。

• 发表时间: 2012
• 作者: Shigeru OKUHARA;Ryosuke NAGAOKA,Takanori AMANO;Tomoko SAGAI;ToshihikoSHIROISHI;Sachiko ISEKI;田野智之;Shigeru Okuhara;Yuta Shibamoto;Shigeru OKUHARA
• 通讯作者: Shigeru OKUHARA