Funactional Analysis of a novel poly-amino acid synthesizing enzyme for synthesis of a desired oligopeptide.

近年来，多肽有望成为功能性食品或药物的原料。在本研究中，我们旨在分析多氨基酸合成酶及其合成机理，并对多种新型多氨基酸生产微生物进行了分离和鉴定。然后对一个编码多氨基酸合成酶的基因进行了表征。通过带电染料筛选和MALDI-TOF质谱分析，获得了多种新的产氨基酸微生物，其中含有一种新的碱性产氨基酸微生物。此外，还分析了一个编码nprs样酶的基因。

Recently, peptides are expected as materials of functional foods or medicine. In this research, we aim to analyze the poly-amino acid synthesizing enzymes and their synthesizing mechanisms, isolation and characterization of various new poly-amino acid producing microorganisms were performed. And then a gene encoding poly-amino acid synthesizing enzyme was characterized. Through the screening with a charged dye and MALDI-TOF MS analysis, various new poly-amino acid producing microorganisms containing a new basic poly-amino acid producing microorganism were obtained. Moreover, a gene encoding NPRS-like enzyme was analyzed.