Preparation of synaptosome derived from specified neural circuit

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The cerebral cortex consists of various types of neural cells which are interconnected and make up a complex network. Therefore, it is necessary to examine the behavior of functional molecules at specified neural connections to understand the function, development and plasticity of the cerebral cortex. The aim of the present project is to prepare the synaptosome sample derived from the neural connections that was fluorescently labeled in advance. To this end, I labeled the neurons in the visual cortex by injecting fluorescent tracers, prepared synaptosome samples from the labeled tissue, and sorted them using flowcytometry according to their fluorescent intensity. I could obtained the successfully labeled synaptosomes from cortical tissue injected with a carbocyanine dye DiI.