Integrated in vit-roin vivo organization of liver tissues having a functional bile duct network

具有功能性胆管网络的肝组织的体外整合组织

• 批准号: 22650108
• 负责人: SAKAI Yasuyuki
• 金额: $ 2.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650108/
• 关键词: 細胞・組織工学; マクロ組織極性; 移植・再生医療; 再生医学; 細胞・組織; 生物・生体工学; 肝細胞; 胆管上皮細胞; 球状凝集体; 胆管; 組織工学; 極性; スフェロイド; 胆管ネットワーク

To organize implantable liver tissues having a functional bile duct network, first, we made spherical cellular aggregates constituted of fetal rat liver cells and mature bile duct epithelial cells isolated from rats. Aggregates having a continuous and functional bile duct network over the entire aggregates were successfully formed in the aggregates containing a three times larger number of mature bile duct cells over fetal liver cells. When such aggregates were immobilized in poly-L-lactic acid scaffolds with Matrigel and were implanted into the mesentery of immune-deficient mice, bile duct networks were well maintained in the case of aggregates having mature bile duct cells, whereas they were not formed in the case of pure fetal liver cell aggregates. These results showed that mature bile duct cells are necessary in forming functional bile duct networks over organized liver tissues.

为了组织具有功能性胆管网络的可植入肝组织，首先，我们制备了由胎鼠肝细胞和从大鼠分离的成熟胆管上皮细胞构成的球形细胞聚集体。在整个聚集体上具有连续和功能性胆管网络的聚集体成功地形成在含有比胎肝细胞大三倍的成熟胆管细胞的聚集体中。当这样的聚集体被固定在聚-L-乳酸支架与基质胶，并被植入到免疫缺陷小鼠的肠系膜，胆管网络得到很好的保持在具有成熟的胆管细胞的聚集体的情况下，而它们没有形成在纯胎肝细胞聚集体的情况下。这些结果表明，成熟的胆管细胞是必要的，在组织的肝组织形成功能性胆管网络。

项目成果

異所性体内埋め込み型人工肝臓開発の現状と展望

异位植入式人工肝的发展现状及前景

• 发表时间: 2010
• 期刊: 生産研究
• 作者: 勝田毅;小森喜久夫;寺谷工;落谷孝広;酒井康行
• 通讯作者: 酒井康行

間葉系幹細胞を用いた細胞治療の可能性

使用间充质干细胞进行细胞治疗的可能性

• 发表时间: 2010
• 期刊: 医工学治療
• 作者: 勝田毅;酒井康行;落谷孝広
• 通讯作者: 落谷孝広

Fetal Tissue Transplantation in Fetal Tissue Transplantation（分担執筆）,

胎儿组织移植中的胎儿组织移植（合著者），

• 发表时间: 2013
• 作者: Y. Sakai1;J. Jiang;S. Hanada;H. Huang;T. Katsuda;N. Kojima;T. Teratani;A. Miyajima;T. Ochiya
• 通讯作者: T. Ochiya

Hypoxia efficiently induces differentiation of mouse embryonic stem cells into endodermal and hepatic progenitor cells

• DOI: 10.1016/j.bej.2013.02.012
• 发表时间: 2013-05-15
• 期刊: BIOCHEMICAL ENGINEERING JOURNAL
• 影响因子: 3.9
• 作者: Katsuda, Takeshi;Teratani, Takumi;Sakai, Yasuyuki
• 通讯作者: Sakai, Yasuyuki