Observations and Analysis of Rotary Movement of a Supramolecular Motor by Single-Molecular Imaging through Actual Time.

肌纤维、鞭毛、纤毛等生物分子马达在自然界中是精确设计的，令人联想到微尺度的电子和机械装置。受直接观察旋转分子马达系统的启发，一些研究者尝试直接观察人工分子马达。相反，即使是更小的人工分子马达也很难看到，因此，需要额外的可视化技术来观察旋转行为。轮烷具有构建分子马达的合适结构。本文观察了基于α-环糊精（α-CD）的单轮烷分子附着在玻璃基板上的荧光探针上，并利用全内反射荧光显微镜（TIRFM）测量了α-CD作为转子的旋转运动。在这里，我们观察的旋转运动

Biological molecular motors such as muscle fibers, flagella and cilia are precisely designed in nature, and are reminiscent of microscale electronic and mechanical devices. Inspired by direct observations of rotary molecular motor systems, some researchers have tried to directly observe artificial molecular motors. On the contrary, even smaller artificial molecular motors are extremely difficult to see, and thus, additional visualization techniques are necessary to observe rotational behavior. Rotaxane has a suitable structure to construct molecular motors. Herein we observe a single rotaxane molecule based onα-cyclodextrin(α-CD) attached to a fluorescence probe on glass substrates and estimate the rotary movement ofα-CD as a rotor, which was measured by total internal reflection fluorescence microscopy(TIRFM). Herein, we observe the rotary movement of