Identification of new determinants for double fertilization

鉴定双受精的新决定因素

• 批准号: 22657017
• 负责人: IGAWA Tomoko
• 金额: $ 2.29万
• 依托单位: Chiba University (2011-2012)The Institute of Physical and Chemical Research (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657017/
• 关键词: 重複受精; 配偶子細胞; ライブイメージング; 配偶子膜; 卵細胞膜

Arabidopsis marker lines, where gamete membrane was specifically visualized with fluorescent protein (GFP), were produced. Membrane proteins were purified from pistil tissue of the egg cell membrane- marker line by pull-down strategy using anti-GFP antibody. Among the identified proteins with putative trans-membrane region, one protein was found to be involved in seed fertility.

产生拟南芥标记系，其中配子膜用荧光蛋白（GFP）特异性地可视化。通过使用抗GFP抗体的下拉策略从卵细胞膜标记系的雌蕊组织纯化膜蛋白。在已鉴定的具有跨膜区的蛋白质中，发现一种蛋白质与种子育性有关。

项目成果

Live cell imaging analysis of gamete membrane behavior during double fertilization of Arabidopsis

拟南芥双受精过程中配子膜行为的活细胞成像分析

• 发表时间: 2012
• 作者: 野々村麻衣子;石崎公庸;大和勝幸;河内孝之;河内孝之;河内孝之;Tomoko Igawa
• 通讯作者: Tomoko Igawa