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Study of Catalytic Mechanism of Hydrogen Activation by Hydrogenase using Neutron Crystal Structure Analysis

Study of Catalytic Mechanism of Hydrogen Activation by Hydrogenase using Neutron Crystal Structure Analysis
中子晶体结构分析研究氢化酶氢活化催化机制
批准号:
22657031
负责人:
HIGUCHI Yoshiki
金额:
$2.19万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011

项目摘要

项目成果

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相关文献

中文摘要
翻译
2010年首次尝试将硫酸盐还原菌接种于氘代培养基中制备完全氘代的[NiFe]氢化酶,但未能获得足够量的蛋白样品用于结晶试验。在2011-2012年,尝试在氘代缓冲溶液中使用氘代沉淀剂结晶普通(非氘代)氢化酶。我们已经成功地制备了体积为4 mm^3的单晶,该晶体在100 K下用日本原子能研究所的中子束给出了分辨率为5.0 A的中子衍射图。此外,还找到了[NiFe]氢化酶大单晶低温衍射实验的一些参数。
英文摘要
The perfectly deuterated[NiFe] hydrogenase was tried to prepare by inoculating the sulfate-reducing bacterium in the deuterated media in 2010.However, it was not successful to obtain enough amount of the protein sample for crystallization trial. In 2011-2012, the ordinary(not deuterated) hydrogenase was tried to crystallize using deuterated precipitant in deuterated buffer solution. We have succeeded in preparation of a single crystal with a volume of 4 mm^3.This crystal gave a neutron diffraction pattern at 5.0 Aresolution at 100 K using the neutron beam at Japan Atomic Energy Research Institute. In addition, some parameters for diffraction experiment for large single crystals of[NiFe] hydrogenase at cryo-temperature have been found.
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マルチ銅酸化酵素の構造解析
多种铜氧化酶的结构分析
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [小森博文, 等]
通讯作者:
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Wnt信号传导机制中CCD1的结构化学研究
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [寺脇慎一, 塩見健輔, 桝正幸, 柴田直樹, 樋口芳樹]
通讯作者: 樋口芳樹
Crystallization and Preliminary X-ray Analysis of NADH:rubredoxin Oxidoreductase from Clostridium acetobutylicum
丙酮丁醇梭菌 NADH:红氧还蛋白氧化还原酶的结晶和初步 X 射线分析
DOI: 10.1107/s1744309109047162
发表时间: 2010
期刊: Acta Crystallogr
影响因子: --
作者: [K. Nishikawa, Y. Shomura, S. Kawasaki, Y. Niimura and Y. Higuchi]
通讯作者: Y. Niimura and Y. Higuchi
Purification, Crystallization and Preliminary X-ray Analysis of the Dissimilatory Sulfite Reductase from Desulfovibrio vulgaris Miyazaki F
普通脱硫弧菌 Miyazaki F 异化亚硫酸还原酶的纯化、结晶和初步 X 射线分析
DOI: 10.1107/s1744309110033191
发表时间: 2010
期刊: Acta Crystallogr.
影响因子: --
作者: [H. Ogata, Y. Shomura, A.G. Agrawal, A.P. Kaur, W. Gartner, Y. Higuchi and W. Lubitz]
通讯作者: Y. Higuchi and W. Lubitz
32
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    • 资助金额:
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    • 批准号:
      22370061
    • 项目类别:
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    • 资助金额:
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    • 财政年份:
      2010
    • 负责人:
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    Activation mechanism of the Active Site of [NiFe] hydrogenas
    • 批准号:
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    • 项目类别:
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    • 资助金额:
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