Development of a novel genome mapping method by using homologous recombination protein

利用同源重组蛋白开发新的基因组作图方法

• 批准号: 22657044
• 负责人: MAESHIMA Kazuhiro
• 金额: $ 2.25万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657044/
• 关键词: 核酸; ゲノム; 生体分子

To label a certain genomic region in the cell, a harsh condition to denature DNA is required. In this proposal, we tried to target a fluorescently tagged DNA sequence to the genomic DNA using some homologous recombination proteins. However, with homologous recombination protein RecA or XRad51, we did not get an efficient targeting in the fixed cells. As an alternative, we designed some fluorescent polyamides, which can recognize a certain DNA sequence, and succeeded to target efficiently the telomere repeats in the fixed cells

为了标记细胞中的某个基因组区域，需要使DNA变性的苛刻条件。在这个方案中，我们尝试使用一些同源重组蛋白将荧光标记的DNA序列靶向到基因组DNA。然而，使用同源重组蛋白RecA或XRad51，我们在固定的细胞中没有得到有效的靶向。作为一种替代方法，我们设计了能够识别特定DNA序列的荧光聚酰胺，并成功地靶向固定细胞中的端粒重复序列

项目成果

The integrator complex is required for the integrity of Cajal bodies

积分器复合体对于 Cajal 体的完整性是必需的

• DOI: 10.1242/jcs.090837
• 发表时间: 2012
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: Fumihiko Maeda;Hiroki Hibino;H.Takata
• 通讯作者: H.Takata

Human genome organization and dynamics

人类基因组组织和动力学

• 发表时间: 2012
• 作者: Y.Kubo;T.Takeuchi;K.Okano;T.Okano;深澤裕貴;前島一博;Kazuhiro Maeshima;Kazuhiro Maeshima
• 通讯作者: Kazuhiro Maeshima

How is a long strand of genomic DNA organized into chromosome?

长链基因组 DNA 是如何组织成染色体的？

• 发表时间: 2011
• 作者: 馴松直紀;岩楯好昭;原田伊知郎;久保葉子;Kazuhiro Maeshima
• 通讯作者: Kazuhiro Maeshima

"Human genome organization and dynamics Paradigm Innovation in Biology

“人类基因组组织和动力学生物学范式创新

• 发表时间: 2012
• 作者: Y.Kubo;T.Takeuchi;K.Okano;T.Okano;深澤裕貴;前島一博;Kazuhiro Maeshima
• 通讯作者: Kazuhiro Maeshima

Chromatin structure: does the 30-nm fibre exist in vivo?

• DOI: 10.1016/j.ceb.2010.03.001
• 发表时间: 2010-06-01
• 期刊: CURRENT OPINION IN CELL BIOLOGY
• 影响因子: 7.5
• 作者: Maeshima, Kazuhiro;Hihara, Saera;Eltsov, Mikhail
• 通讯作者: Eltsov, Mikhail