Development of a novel genome mapping method by using homologous recombination protein

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To label a certain genomic region in the cell, a harsh condition to denature DNA is required. In this proposal, we tried to target a fluorescently tagged DNA sequence to the genomic DNA using some homologous recombination proteins. However, with homologous recombination protein RecA or XRad51, we did not get an efficient targeting in the fixed cells. As an alternative, we designed some fluorescent polyamides, which can recognize a certain DNA sequence, and succeeded to target efficiently the telomere repeats in the fixed cells