Identification of Al-binding peptide

我们的功能特点ART调控基因，OsCDT3。OsCDT3基因编码53个氨基酸残基，其中14个（26. 4%）是半胱氨酸残基。OsCDT3主要在根中表达，且其表达受铝胁迫的特异性诱导。OsCDT3定位于细胞膜。OsCDT3基因的敲除提高了水稻对铝的敏感性。表达OsCDT3的酵母对铝的耐受性增强，铝的积累减少，表明OsCDT3可能在质外体中发挥铝的解毒作用。

We functionally characterized an ART-regulated gene, OsCDT3. OsCDT3 encodes a predicted peptide of 53 amino acid residues, of which 14(26. 4%) are cysteine residues. OsCDT3 was mainly expressed in the roots and its expression was specifically induced by Al exposure. OsCDT3 was localized to the plasma membrane. Knockdown of OsCDT3 resulted in increased sensitivity to Al in rice. Yeast expressing OsCDT3 showed increased tolerance to Al and decreased accumulation of Al. Our results indicate that OsCDT3 may play a role in detoxifying Al at the apoplast.