Establishment of sperm cells in vitro from female mouse somatic cells

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Genomic imprinting is indispensable for the proper mammalian development and is established during gametogenesis. I focused on DNA methylation, which plays an essential role for the establishment of imprinting, to create sperm-like cells from female mouse somatic cells in vitro. Three different types of DNA methylation inhibitors were added to mouse embryonic fibroblast and DNA methylation status was analyzed. There was a decrease of DNA methylation levels in some imprinted genes, however, no inhibitors could induce hypo-methylation in all imprinted genes analyzed.