Development of analytical methods of carbon and nitrogen stable isotope ratio in nucleic acids

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The objective of this project was to organize a novel stable isotope technology for nucleotide especially rRNA in order to develop a novel methodology in environmental omics. In this study, we optimized the online separation and detection for deoxyribonucleotide by using high performance liquid chromatography combined with electrospray ionization mass spectrometry(unpublished). However, we could not construct a new methods for purification of nucleotides to use stable isotopic analysis due to several technical difficulties.