Development of analytical methods of carbon and nitrogen stable isotope ratio in nucleic acids

核酸碳氮稳定同位素比值分析方法的建立

• 批准号: 22658107
• 负责人: NUNOURA Takuro
• 金额: $ 2.34万
• 依托单位: Japan Agency for Marine-Earth Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658107/
• 关键词: 生物環境; 安定同位体; 微生物; 核酸

The objective of this project was to organize a novel stable isotope technology for nucleotide especially rRNA in order to develop a novel methodology in environmental omics. In this study, we optimized the online separation and detection for deoxyribonucleotide by using high performance liquid chromatography combined with electrospray ionization mass spectrometry(unpublished). However, we could not construct a new methods for purification of nucleotides to use stable isotopic analysis due to several technical difficulties.

本项目的目标是建立一种新的核苷酸特别是rRNA的稳定同位素技术，以发展一种新的环境组学方法。在这项研究中，我们优化了在线分离和检测脱氧核糖核苷酸的高效液相色谱电喷雾质谱（未发表）。然而，由于技术上的困难，我们无法建立一种新的纯化核苷酸的方法，使用稳定同位素分析。