Development of analytical methods of carbon and nitrogen stable isotope ratio in nucleic acids

本项目的目标是组织一种新的核苷酸特别是rRNA稳定同位素技术，以开发一种新的环境组学方法。在本研究中，我们优化了高效液相色谱-电喷雾电离质谱法在线分离检测脱氧核糖核苷酸的方法(未发表)。然而，由于一些技术上的困难，我们无法构建一种新的核苷酸纯化方法来使用稳定同位素分析。

The objective of this project was to organize a novel stable isotope technology for nucleotide especially rRNA in order to develop a novel methodology in environmental omics. In this study, we optimized the online separation and detection for deoxyribonucleotide by using high performance liquid chromatography combined with electrospray ionization mass spectrometry(unpublished). However, we could not construct a new methods for purification of nucleotides to use stable isotopic analysis due to several technical difficulties.