Development of asbestos related tumor marker using protein array methods

应用蛋白质芯片技术开发生物标志物检测系统。用手动点样器对人血浆样本进行斑点，并应用HRP标记的抗间皮蛋白抗体。该系统检测C末端的蛋白质，而现有的ELISA使用针对N和C末端的两种抗体。这两种方法的结果并不相互关联。目前正在使用包括放射学结果在内的其他临床数据进行进一步分析。还需要优化蛋白质阵列测量以减少处理时间。

Protein array method was applied to develop biomarker measurement system. Manual arrayer was used to spot human plasma sample and HRP labeled anti-mesothelin antibody was applied. The system detects the protein at C terminal, while the available ELISA uses two antibodies for N and C terminals. The results from the two methods did not correlate each other. Further analysis using other clinical data including radiological findings is now underway. Optimization of protein array measurement to reduce processing time is also needed.