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A research about the similarity with an organization stem cell and the cancer

关于组织干细胞与癌症相似性的研究

基本信息

批准号：
22659310
负责人：
YOKOO Seiichi
金额：
$ 2.09万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

项目摘要

It was difficult to obtain the cancer which broke out on the surface of eyes. Therefore I searched the cell line, but there is not the cell line derived from the surface of eyes. Therefore I performed various kinds of examination that should have accumulated data of the normal corneal epithelial stem cell which would become Control Data in the future. I examined the growth factor which an increase effect was reported in to a corneal epithelium. The increase of the corneal epithelium understood(TGFα),(HGF),(KGF) other than EGF that an effect was high. When I used EGF, KGF, TGFα, the manufacture of the corneal epithelium tissue was possible when it for three weeks.When I make a corneal epithelium-like organization using EGF, there were not undifferentiated candidate marker, cytokeratin 15(CK15) expressed to a cornea limbus epithelium. The corneal epithelium form that multiplied becomes flatness a little in EGF, but, in the corneal epithelium-like organization which manufactured it only by KGF addition, a basal cell is columnar. Therefore expression of undifferentiated marker CK15 which developed in cornea limbus came to be seen in the corneal epithelium-like cell sheet which I made in the culture fluid which added only KGF when I examined undifferentiated marker CK15. The other undifferentiated marker candidates examined, too. As for the corneal epithelium sheet derived from a stem cell cultured using EGF, expression is not seen in them at all. The expression of various undifferentiated markers was seen by addition of KGF including p63, p75NTR, N-Cad. KGF strongly participating in property maintenance of the epithelium stem cell than these results is thought about. Again than a fact of these marker expression, It is thought that I could make a cornea limbus epithelium cell sheet by addition of KGF and am suitable for transplant more.

很难获得在眼睛表面爆发的癌症。因此，我搜索了细胞系，但没有来自眼睛表面的细胞系。因此，我进行了各种检查，这些检查应该积累了正常角膜上皮干细胞的数据，这些数据将成为未来的对照数据。我检查了生长因子，据报道它对角膜上皮有增加作用。角膜上皮的增加理解为（TGF α）、（HGF）、（KGF）而不是EGF，其效果高。当我用EGF、KGF、TGF α制作角膜上皮组织时，当我用EGF制作角膜上皮样组织时，没有未分化的候选标记物细胞角蛋白15（CK 15）表达到角膜利姆布斯上皮。在EGF中增殖的角膜上皮形式变得平坦一点，但是，在仅由KGF添加制造的角膜上皮样组织中，基底细胞是柱状的。因此，在角膜利姆布斯中发育的未分化标记物CK 15的表达在角膜上皮样细胞片中被观察到，当我检查未分化标记物CK 15时，我在仅添加KGF的培养液中制备了角膜上皮样细胞片。其他未分化的候选标记也进行了检查。至于由使用EGF培养的干细胞衍生的角膜上皮片，在它们中根本看不到表达。加入KGF后，可观察到各种未分化标志物的表达，包括p63、p75NTR、N-Cad。KGF强烈参与上皮干细胞的性质维持，而不是这些结果被认为。再加上这些标志物的表达，我们认为通过添加KGF可以使角膜利姆布斯上皮细胞片更适合移植。