Development of Novel Fluorogenic Probes for Labeling Intracellular Proteins

通过一对特定的蛋白质标签和合成探针标记荧光蛋白质是研究活细胞内蛋白质功能和定位的一种强有力的化学方法。在本研究中，我们开发了一种基于荧光探针和光活性黄蛋白(PYP)标签的蛋白质标记系统。利用这一技术，我们成功地对活细胞内的蛋白质进行了特异性成像，获得了高的S/N比和良好的标记动力学。此外，将该标记技术应用于DNA甲基化的成像。

Fluorescence protein labeling by a specific pair of a protein tag and synthetic probe is a powerful chemistry-based approach to investigate protein function and localization inside living cells. In this research, we developed a protein labeling system based on fluorogenic probes and photoactive yellow protein (PYP) tag. By utilizing this technique, we were succeeded in specific imaging of proteins inside living cells with high S/N ratio and excellent labeling kinetics. Furthermore, the labeling technique was applied to the imaging of DNA methylation.